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Mourino-Perez R, Linacre-Rojas L, Roman-Gavilanes A, Lew T, Callejas-Negrete O, Roberson R, Freitag M
Wikimedia Commons
Microtubules dynamics (β-tubulin-GFP) in the WT.
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Discover the microscopic world of ascomycete fungi as we speak with Sabine Huhndorf, Assistant Curator of Fungi and Lichens in the Botany Department at the Field Museum of Natural History.
Science at FMNH is a podcast and video series that explores the behind-the-scenes science, collections and research at Chicago's Field Museum.
[taxonomy:class= Dothideomycetes]
[taxonomy:class= Sordariomycetes]
[taxonomy:kingdom= Fungi]
[taxonomy:phylum= Ascomycota]
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We continue our discussion with Sabine Huhndorf by discussing how ascomycete fungi can reproduce both sexually and asexually.
Science at FMNH is a podcast and video series that explores the behind-the-scenes science, collections and research at Chicagoâs Field Museum.
[taxonomy:class= Dothideomycetes]
[taxonomy:class= Sordariomycetes]
[taxonomy:kingdom= Fungi]
[taxonomy:phylum= Ascomycota]
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Defects in sbg1 Δ cells are partially rescued by sorbitol. sbg1Δ spores with ring marker Rlc1-tdTomato were dissected and germinated on YE5S + 1.2 M sorbitol, imaged 24 h after tetrad dissection with a time interval of 10 min. Time is in h: min. Frame rate: 7 fps.
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Canonici A, Siret C, Pellegrino E, Pontier-Bres R, Pouyet L, Montero M, Colin C, Czerucka D, Rigot V, André F
Wikimedia Commons
HCT-8/E11 cell monolayers were wounded as described in Methods and incubated with Sb supernatant. Plates were placed in a temperature and CO2-controlled chamber mounted on a Nikon TE2000 inverted microscope. Images were captured every 5 minutes for a total observation time of 5 hours using a Cool SnapHQ camera (Princeton Instrument) through a 10× objective lens.
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Mouriño-Pérez R, Linacre-Rojas L, Román-Gavilanes A, Lew T, Callejas-Negrete O, Roberson R, Freitag M
Wikimedia Commons
TIRFM of MTB-3-GFP cortical comets dynamics in mature hypha of N. crassa .
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We continue our discussion with Sabine Huhndorf by exploring the different stages of decomposition and the role of ascomycete fungi in this process.
Science at FMNH is a podcast and video series that explores the behind-the-scenes science, collections and research at Chicagoâs Field Museum.
[taxonomy:class= Dothideomycetes]
[taxonomy:class= Sordariomycetes]
[taxonomy:kingdom= Fungi]
[taxonomy:phylum= Ascomycota]
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Ettrich R, Kopecký V, Hofbauerová K, Baumruk V, Novák P, Pompach P, Man P, Plíhal O, Kutý M, Kulik N, Sklenář J, Ryšlavá H, Křen V, Bezouška K
Wikimedia Commons
Molecular dynamics simulation of β-N-hexosaminidase with bound chitobiose. The movie shows 1 ns of simulation of the equilibrated structure with chitobiose docked into the active site (non-reducing sugar: green; reducing sugar: white). Additionally, the catalytic amino acid Glu346 and amino acids Asp345 (pink) and Trp482 (orange) are shown as stick models. The disulfide bridge connecting the large flexible loop (magenta) is shown in yellow.
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Mouriño-Pérez R, Linacre-Rojas L, Román-Gavilanes A, Lew T, Callejas-Negrete O, Roberson R, Freitag M
Wikimedia Commons
MTB-3-GFP comets dynamics in mature hypha of N. crassa. Comets are distributed homogeneously along the hypha and moved in anterograde and retrograde direction.
-
Discover the microscopic world of ascomycete fungi as we speak with Sabine Huhndorf, Assistant Curator of Fungi and Lichens in the Botany Department at the Field Museum of Natural History.
Science at FMNH is a podcast and video series that explores the behind-the-scenes science, collections and research at Chicago's Field Museum.
[taxonomy:class= Dothideomycetes]
[taxonomy:class= Sordariomycetes]
[taxonomy:kingdom= Fungi]
[taxonomy:phylum= Ascomycota]
-
Mourino-Perez R, Linacre-Rojas L, Roman-Gavilanes A, Lew T, Callejas-Negrete O, Roberson R, Freitag M
Wikimedia Commons
Microtubules dynamics (β-tubulin-GFP) in the mtb-3 gene deletion mutant.
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Time-course of YFP-CDC-42 localization during lateral branch initiation.
-
We continue our discussion with Sabine Huhndorf by discussing how ascomycete fungi can reproduce both sexually and asexually.
Science at FMNH is a podcast and video series that explores the behind-the-scenes science, collections and research at Chicagoâs Field Museum.
[taxonomy:class= Dothideomycetes]
[taxonomy:class= Sordariomycetes]
[taxonomy:kingdom= Fungi]
[taxonomy:phylum= Ascomycota]
-
Mourino-Perez R, Linacre-Rojas L, Roman-Gavilanes A, Lew T, Callejas-Negrete O, Roberson R, Freitag M
Wikimedia Commons
Dynamics of MTB-3-GFP in the UV mutant of the p150glued subunit of dynactin ( ro-3 ).
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We continue our discussion with Sabine Huhndorf by exploring the different stages of decomposition and the role of ascomycete fungi in this process.
Science at FMNH is a podcast and video series that explores the behind-the-scenes science, collections and research at Chicagoâs Field Museum.
[taxonomy:class= Dothideomycetes]
[taxonomy:class= Sordariomycetes]
[taxonomy:kingdom= Fungi]
[taxonomy:phylum= Ascomycota]
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Summary[
edit] Description: English: Cells lacking Gap1 were treated as described for Movie S1. Many aberrant responses were observed upon pheromone treatment including multiple projection tips, enlarged vacuoles and cell lysis (original data for Figure 4A). Date: 2013. Source: Movie S2 from Weston C, Bond M, Croft W, Ladds G (2013). "
The Coordination of Cell Growth during Fission Yeast Mating Requires Ras1-GTP Hydrolysis". PLOS ONE.
DOI:
10.1371/journal.pone.0077487.
PMID 24147005.
PMC:
3797800. Author: Weston C, Bond M, Croft W, Ladds G. Permission (
Reusing this file): : This file is licensed under the
Creative Commons Attribution 2.5 Generic license.:. You are free: to share – to copy, distribute and transmit the work to remix – to adapt the work Under the following conditions: attribution – You must attribute the work in the manner specified by the author or licensor (but not in any way that suggests that they endorse you or your use of the work). http://creativecommons.org/licenses/by/2.5 CC BY 2.5 Creative Commons Attribution 2.5 truetrue. This file was published in a
Public Library of Science journal.
Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.. Provenance: This file was transferred to Wikimedia Commons from
PubMed Central by way of the
Open Access Media Importer.:
.
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Mourino-Perez R, Linacre-Rojas L, Roman-Gavilanes A, Lew T, Callejas-Negrete O, Roberson R, Freitag M
Wikimedia Commons
Dynamics of MTB-3-GFP in the deletion mutant of kin-1 gene (Δ kin-1 ).
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Sbg1 depends on the secretory pathway for its trafficking. Cells expressing mEGFP-Sbg1 were treated with Brefeldin-A (BFA) or its solvent ethanol for ~10 min and imaged on bare slide with a time interval of 1 min. Sbg1 signal in cytoplasmic puncta and cell tips are dramatically reduced. Time is in h: min. Frame rate: 7 fps.
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Time-course of GFP-CDC-24 localization during lateral branch initiation.
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Dynamic distribution of YFP-RAC in mature hyphal tips.
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Sbg1 and Bgs1 co-localize and move together in cytoplasmic vesicles. The strain expressing mEGFP-Sbg1 (green, left) and tdTomato-Bgs1 (red, middle) was grown in YE5S at exponential phase for 36 h before imaging continuously (1.1 frames/sec) at a single focal plane. Time is in min: sec. Frame rate: 7 frames per sec (fps).
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Dynamic distribution of GFP-CDC-24 in mature hyphal tips.
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Time-course of YFP-RAC localization during lateral branch initiation.