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A. Live specimen.-B. Male paired seminal vesicles receive sperm from the copulatory organ, squeezed live specimen.-C. Insemination apparatus, same specimen.
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Section through the proximal bursa (vab) and the ductus spermatici (dsp),?780x
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Fig.2 C. Frontal body part, ho1otype.-D. Male sclerotic structures, holotype. Fig.1D. Male sclerotic organs, holotype
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Cicerina debrae n. sp. Anterior end is to the left in Fig C and to the top in the remaining figures. A. General anatomy; arrows, adhesive belts; br, brain; cb, copulatory bulb; dsp, ductus spermaticus; ge, germarium; go, common gonopore; ph, pharynx; sv, seminal vesicle; t, testes; v, vitellaria. B. Bright-field micrograph of a specimen from a resinslide preparation, showing genital region with paired germaria, ducti spermatici with flanges where they enter the bursal tissue (bu), proximal prostatic region of copulatory bulb (pr), and distal portion of copulatory bulb with cirrus (ci) and matrix-cell nucleus (arrow). C. CLSM stack (Alexa488/phalloidi?green; Hoechst 3334?blue) of genital region, lateral view showing seminal vesicle, proximal region of copulatory bulb with invaginated internal ductus ejaculatorius (de), distal (di) region of copulatory bulb with separate muscle layer, and vagina interna (vi) ending in a sphincter (sph) at the bursal tissue. D, E. Brightfield micrographs of same specimen as B, more-dorsal and more-ventral (respectively) focal planes of the cirrus, showing arrangement of spines, and hemi-circular wreath of gland cells (gl) located at the base of the cirrus.
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A. Genital organs in part, live specimen.-B. Male sclerotic structures, ho1otype.-
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A. Free-crawling animal, B. Copulatory organ on a squeezed animal, C. Bursal piece on a squeezed animal, D. Caudal ends of live animals, E. Reconstruction of the genital organs as seen from below, from horizontal sections, F. Cirrus in sagital section, G. Copulatory organ, uterus and bursa from a horizontal section. A, B und D free-hand drawings, all other figures with camera lucida: C 650 x, E 500 x, F and G 950 X.
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135. Organization (live specimens), 136. Spines of the copulatory organ (squeezed animal), 137. Bursal pieces (squeezed animal)
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insemination tube, ho1otype.
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A. Organisation, from observations on live animals, Kieler Bucht. B. Copulatory organ with curved cirrus. Kieler Bucht. C. Ductus spermatici (bursal pieces) from an animal from the Kieler Bucht. Surendorf. D. The same, from an individual from the bay of Arcachon. Ile aux Oiseaux.
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14. Slightly squeezed live animal, 15. cirrus spines, 16. cuticular sprematic ducts (15 and 16 at the same scale)
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Drawing of living animal
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135. Organization (live specimens), 136. Spines of the copulatory organ (squeezed animal), 137. Bursal pieces (squeezed animal)
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Drawing of living animal
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E. Reconstruction of the genital organs as seen from below, from horizontal sections, F. Cirrus in sagital section, G. Copulatory organ, uterus and bursa from a horizontal section. All with camera lucida: E 500 x, F and G 950 X.
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Drawing of living animal
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A. Free-crawling animal, free-hand drawing.
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Drawing of living animal
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B. Copulatory organ on a squeezed animal, C. Bursal piece on a squeezed animal, D. Caudal ends of live animals
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Schematic sagital section through the proboscis, 610x. Two gland cells of the proboscis cavity are sectioned tangentially. Proboscis cone retracted. np = nerve plexus
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Transverse section through the bursa (vab) and the ductus spermatici (dsp),?780x
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Sagital reconstruction of the genital system,?310x
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Dorsal view of a live animal
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A. Caudal end (free-hand drawing). B. Bursal piece on a squeezed animal (with camera lucida, 1060x).