Heterodera filipjevi with Monocillium gamsii (10.3897-mycokeys.27.21254) Figure 4

Description:

Figure 4; Cysts and eggs of Heterodera filipjevi infected by Monocillium gamsii (= Niesslia gamsii) exhibiting colonisation in vitro. A, B infected cysts rendered black-dotted due to fungal-colonised eggs containing microsclerotia. C, D Eggs with mature sclerotia, extracted from symptomatic cysts. E, F Individual hyphae penetrating eggshell (arrows indicate the individual hyphae; V indicates vacuole-like structures). G–M Fungal development inside the eggs. G, H Earlier stages of infection in unembryonated eggs. I, J Fungal development in the body cavity of developing juveniles where enlarged, thick-walled cells are formed and coalesce to initiate microsclerotia formation. K–M Microsclerotia developing to full size and pigmentation. N–P Pigmentation in microsclerotia from pale-olivaceous to darkly brown. Scale bars: 600 µm (A); 300 µm (B); 30 µm (C–O); 50 µm (P).